DNA binding property, nuclease activity and cytotoxicity of Zn(II) complexes of terpyridine derivatives |
| |
Authors: | Qin Jiang Jianhui Zhu Yangmiao Zhang Nan Xiao Zijian Guo |
| |
Institution: | (1) Chemistry Department, Huaihai Institute of Technology, Lianyungang, 222005, People’s Republic of China;(2) State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing, 210093, People’s Republic of China |
| |
Abstract: | Two zinc(II) terpyridine complexes Zn(atpy)2(PF6)2 (1) (atpy = 4′-p-N9′-adeninylmethylphenyl-2,2′:6,2′′-terpyridine) and Zn(ttpy)2(PF6)2 (2) (ttpy = 4′-p-tolyl-2,2′:6,2′′-terpyridine) have been synthesized and characterized by elemental analysis, 1H NMR and electrospray mass spectroscopy. The structure of complex 2 was also determined by X-ray crystallography, which revealed a ZnN6 coordination in an octahedral geometry with two terpyridine acting as equatorial ligands. The circular dichroism data showed
that complex 1 exhibited an ICD signal at around 300 nm and induced more evident disturbances on DNA base stacking than complex 2, reflecting the impact of the adenine moiety on DNA binding modes. Complex 1 exhibited higher cleavage activity to supercoiled pUC 19 DNA than complex 2 under aerobic conditions, suggesting a promotional effect of adenine moiety in DNA nuclease ability. Interestingly, both
complexes demonstrated potent in vitro cytotoxicity against a series human tumor cell lines such as human cervix carcinoma
cell line (HeLa), human liver carcinoma cell line (HepG2), human galactophore carcinoma cell line (MCF-7) and human prostate
carcinoma cell line (pc-3). The cytotoxicity is averagely 10 times more active than the anticancer drug cisplatin.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
| |
Keywords: | Adenine DNA binding DNA cleavage Terpyridine Zinc |
本文献已被 SpringerLink 等数据库收录! |
|