Induction of antibody-producing cells in rainbow trout, Salmo gairdneri Richardson; by flush exposure |
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| Authors: | D P Anderson B S Roberson O W Dixon |
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| Institution: | U.S. Fish and Wildlife Service, National Fish Health Research Laboratory, Route 3, Box 50, Kearneysville, West Virginia, 25430, U.S.A.; Department of Microbiology, University of Maryland, College Park, Maryland, 20742, U.S.A.; U.S. Fish and Wildlife Service, National Fish Health Research Laboratory, Route 3, Box 50, Kearney sville, West Virginia 25430 |
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| Abstract: | Splenic antibody-producing cells were produced by rainbow trout that had been exposed to O-antigens extracted from Yersinia ruckeri and Aeromonas salmonicida by adding the concentrated antigen preparation directly into the water of the tank holding the fish for a flush exposure. This method was compared with the proven techniques of exposure: intraperitoneal injection or a 2 minute immersion of the fish in the antigen preparation. Dosage experiments showed that the production of antibody-producing cells was induced by the immersion of trout for 2 minutes in water with 5.0 μg/ml-1 (or more) with the Y. ruckeri O-antigen, or 500 μg ml-1 (or more) of the A. salmonicida O-antigen. Similar differences were evident when the respective antigens were added directly to the water. |
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