Catalytic Mechanisms of Fe(II)- and 2-Oxoglutarate-dependent Oxygenases |
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Authors: | Salette Martinez Robert P. Hausinger |
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Affiliation: | From the Departments of ‡Microbiology and Molecular Genetics and ;§Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824 |
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Abstract: | Mononuclear non-heme Fe(II)- and 2-oxoglutarate (2OG)-dependent oxygenases comprise a large family of enzymes that utilize an Fe(IV)-oxo intermediate to initiate diverse oxidative transformations with important biological roles. Here, four of the major types of Fe(II)/2OG-dependent reactions are detailed: hydroxylation, halogenation, ring formation, and desaturation. In addition, an atypical epimerization reaction is described. Studies identifying several key intermediates in catalysis are concisely summarized, and the proposed mechanisms are explained. In addition, a variety of other transformations catalyzed by selected family members are briefly described to further highlight the chemical versatility of these enzymes. |
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Keywords: | dioxygenase enzyme mechanism hydroxylase iron metalloenzyme 2-oxoglutarate desaturation halogenase ring-formation |
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