Minimally-invasive Technique for Injection into Rat Optic Nerve |
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Authors: | Kateryna Raykova Melina V Jones Hwa Huang Paul F Hoffman Michael Levy |
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Affiliation: | 1.Department of Neurology, Johns Hopkins University |
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Abstract: | The rat optic nerve is a useful model for stem cell regeneration research. Direct injection into the rat optic nerve allows delivery into the central nervous system in a minimally-invasive surgery without bone removal. This technique describes an approach to visualization and direct injection of the optic nerve following minor fascial dissection from the orbital ridge, using a conjunctival traction suture to gently pull the eye down and out. Representative examples of an injected optic nerve show successful injection of dyed beads. |
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Keywords: | Neuroscience Issue 99 optic nerve injection stem cells regeneration optic neuritis optic neuropathy |
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