PEGylated chitosan complexes DNA while improving polyplex colloidal stability and gene transfection efficiency |
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| Institution: | 1. Biomechanics, Department of Structural Engineering, NTNU, Norwegian University of Science and Technology, Trondheim 7491, Norway;2. Biophysics and Medical Technology, Department of Physics, NTNU, Norwegian University of Science and Technology, Trondheim 7491, Norway;1. Departments of Polymer Science and Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea;2. Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea;3. Bio Research Center, Samsung Advanced Institute of Technology, Yongin 446-712, Republic of Korea;4. Department of Health Sciences and Technology, Sungkyunkwan University, Suwon 440-746, Republic of Korea |
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| Abstract: | Chitosan is widely explored as a gene delivery vehicle due to its ability to condense DNA, facilitate transport, and subsequent release allowing gene expression, as well as protecting the DNA. Here, we investigate the enhancement of chitosan–DNA dispersion stability while maintaining transfection efficacy by PEGylation of chitosan. Molecular properties of fully deacetylated chitosans and degree of PEGylation were investigated with respect to compaction of DNA, stability and transfection efficacy. Each of the three chitosan samples with varying chain lengths was PEGylated at three different degrees. The chitosans with degree of PEGylation from 0.6 to 1.9% made polyplexes with DNA. PBS induced colloidal aggregation of polyplexes with initial radius of about 100 nm observed for nonPEGylated chitosans was suppressed for 1.9% PEGylated chitosans. The observed increase in transfection efficacy coinciding with increased polyplex colloidal stability suggests that aggregation of gene-delivery packages may reduce the transfection efficacy. |
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