Mitochondrial fusion in Chlamydomonas reinhardtii zygotes |
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| Institution: | 1. Bielefeld University, Faculty of Biology, Center for Biotechnology (CeBiTec), Universitätsstrasse 27, 33615 Bielefeld, Germany;2. Liège University, Genetics and Physiology of Microalgae, Chemin de la Vallée 4, 4000 Liège 1, Belgium;3. Liège University, Functional Genomics and Plant Molecular Imaging, Center for Protein Engineering (CIP), Chemin de la Vallée 4, 4000 Liège 1, Belgium;4. Liège University, Centre for Assistance in Technology of Microscopy (CATM), Department of Chemistry, 4000 Liège 1, Belgium;1. Key Laboratory of Geographic Information Science, Ministry of Education, School of Geographic Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China;2. Laboratory of Aquaculture Nutrition and Environmental Health (LANEH), School of Life Sciences, East China Normal University, 200241 Shanghai, China;3. State Key Laboratory of Estuarine and Coastal Research, East China Normal University, 3663 Zhongshan North Road, Shanghai 200062, China;1. College of Fisheries, Huazhong Agricultural University, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, China;2. Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China;3. Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China |
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| Abstract: | Mitochondria are dynamic organelles that were found to fuse and divide in many different cell types. Mitochondrial fusion plays important roles in maintenance of respiratory capacity, dissipation of metabolic energy, and inheritance of mitochondrial DNA. While the molecular machinery of mitochondrial fusion has been characterized in great detail in yeast and mammals, only little is known about mitochondrial fusion in higher plants and algae. We asked whether mitochondrial fusion can be observed in the unicellular green alga Chlamydomonas reinhardtii. Mitochondria were stained with fluorescent dyes in gametes, and mixing of fluorescent markers was detected by fluorescence microscopy in zygotes indicating fusion. Mitochondrial fusion was observed in wild type zygotes, and also in respiratory mutants, albeit with less efficiency. We conclude that mitochondria readily fuse in green algae. |
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