Affiliation: | (1) Departamento de Engenharia Biológica, Campus de Gualtar, 4710-057 Braga, Portugal;(2) Laboratoire de Microbiologie UMR UB/INRA 1232, école Nationale Supérieure de Biologie Appliquée à la Nutrition et à l’Alimentation (ENSBANA), 21000 Dijon, France |
Abstract: | Yarrowia lipolytica converts methyl ricinoleate to γ-decalactone, a high-value fruity aroma compound. The highest amount of 3-hydroxy-γ-decalactone produced by the yeast (263 mg l-1) occurred by increasing the kLa up to 120 h−1 at atmospheric pressure; above it, its concentration decreased, suggesting a predominance of the activity of 3-hydroxyacyl-CoA dehydrogenase. Cultures were grown under high-pressure, i.e., under increased O2 solubility, but, although growth was accelerated, γ-decalactone production decreased. However, by applying 0.5 MPa during growth and biotransformation gave increased concentrations of dec−2-en-4-olide and dec-3-en-4-olide (70 mg l−1). |