Natural variation in the promoter of rice calcineurin B‐like protein10 (OsCBL10) affects flooding tolerance during seed germination among rice subspecies |
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Authors: | Fu‐Yuan Zhu Yi‐Zhen Wu Li‐Juan Xie Tie‐Yuan Liu Ze‐Zhuo Su Shi Xiao Hao Zhang Jianchang Yang Hai‐Yong Gu Xuan‐Xuan Hou Qi‐Juan Hu Hui‐Juan Yi Chang‐Xiang Zhu Jianhua Zhang Ying‐Gao Liu |
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Institution: | 1. College of Biology and the Environment, Nanjing Forestry University, Nanjing, Jiangsu Province, China;2. School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong, China;3. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat‐sen University, Guangzhou, China;4. Jiangsu Key Laboratory of Crop Genetics and Physiology/Co‐Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China;5. The Rice Research Institute of Guangdong Academy of Agricultural Sciences (GDRRI), Guangzhou, China;6. State Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Taian, Shandong, China;7. Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China;8. College of Life Sciences, Nantong University, Nantong, Jiangsu, China;9. Department of Biology, Hong Kong Baptist University, and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong, China |
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Abstract: | Rice (Oryza sativa L.) has two ecotypes, upland and lowland rice, that have been observed to show different tolerance levels under flooding stress. In this study, two rice cultivars, upland (Up221, flooding‐intolerant) and lowland (Low88, flooding‐tolerant), were initially used to study their molecular mechanisms in response to flooding germination. We observed that variations in the OsCBL10 promoter sequences in these two cultivars might contribute to this divergence in flooding tolerance. Further analysis using another eight rice cultivars revealed that the OsCBL10 promoter could be classified as either a flooding‐tolerant type (T‐type) or a flooding‐intolerant type (I‐type). The OsCBL10 T‐type promoter only existed in japonica lowland cultivars, whereas the OsCBL10 I‐type promoter existed in japonica upland, indica upland and indica lowland cultivars. Flooding‐tolerant rice cultivars containing the OsCBL10 T‐type promoter have shown lower Ca2+ flow and higher α‐amylase activities in comparison to those in flooding‐intolerant cultivars. Furthermore, the OsCBL10 overexpression lines were sensitive to both flooding and hypoxic treatments during rice germination with enhanced Ca2+ flow in comparison to wild‐type. Subsequent findings also indicate that OsCBL10 may affect OsCIPK15 protein abundance and its downstream pathways. In summary, our results suggest that the adaptation to flooding stress during rice germination is associated with two different OsCBL10 promoters, which in turn affect OsCBL10 expression in different cultivars and negatively affect OsCIPK15 protein accumulation and its downstream cascade. |
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Keywords: | calcium signaling flooding tolerance hypoxia stress natural variation
Oryza sativa
seed germination |
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