A serine protease inhibitor from hemolymph of green mussel, Perna viridis |
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| Authors: | Khan M S Goswami U Rojatkar S R Khan M I |
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| Institution: | aDivision of Biochemical Science, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411 008, India;bNational Institute of Oceanography, Dona Paula, Goa 403004, India;cDivision of Organic Chemistry, National Chemical Laboratory, Pune 411 008, India |
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| Abstract: | Bioactivity guided fractions of cell-free hemolymph of bacterially challenged marine mussel, Perna viridis led to the isolation of a novel quaternary alkaloid 1, which was identified by its spectral data. The isolated molecule 1 has been found to be a potent serine protease inhibitor (SPI) showing IC50 and Ki values of 102.5 and 97.1–104.68 μM, respectively. The Et/Ki value of SPI is 6.3, whereas Et/Km value is 1.04. The Van’t Hoff analysis showed that the value of Ki decreases with increase in temperature, and the binding of the inhibitor is entropically driven. |
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| Keywords: | Perna viridis Protease inhibitor Natural product Hemolymph |
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