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Characterization of rhizospheric bacteria isolated from Deschampsia antarctica Desv.
Authors:Leticia Barrientos-Díaz  Manuel Gidekel  Ana Gutiérrez-Moraga
Affiliation:1. Departamento de Ciencias Básicas, Facultad de Ciencias, Universidad del Bío-Bío, Casilla 447, Chillan, Chile
2. Laboratorio de Biología Molecular Aplicada, Instituto de Agroindustrias, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Casilla 54-D, Temuco, Chile
3. Ejecutivo Vitrogen S.A., Temuco, Chile
4. Facultad de Ingeniería y Ciencias, Universidad Adolfo Ibá?ez, Diagonal Las Torres 2640, Pe?alolén, Santiago, Chile
Abstract:Deschampsia antarctica Desv. is the only gramineae capable of colonizing the Antarctic due to the region’s extreme climate and soil environment. In the present research, bacteria colonizing the rhizospheric soil of D. antarctica were isolated and characterized. The soil studies showed that D. antarctica possesses a wide spectrum of psychrotolerant bacteria with extensive and varied antibiotic resistance, as well as heavy metal tolerance. The bacterial strains isolated from the rhizosphere of D. antarctica also produced a diverse pattern of enzymes. Based on the strain identification with partial characterization of the 16S rRNA gene, the majority of the isolates correspond to different Pseudomonas species, and species of the genus Flavobacterium sp. and Arthrobacter sp. The isolated strains collected from this research constitute a unique collection for future, more detailed taxonomic analysis and physiological characterization, contributing to the search for potential biotechnological uses. These findings and others have great potential for developing new biotechnological products from Antarctic microorganisms.
Keywords:Antarctic  Antarctic bacteria  Heavy metal tolerance  Resistance to antibiotics  16S rRNA
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