Dehydrogenation of a phosphonate substrate analogue by glycerol 3-phosphate dehydrogenase |
| |
| Authors: | Paul R. Adams Roger Harrison Thomas D. Inch |
| |
| Affiliation: | Biochemistry Group, School of Biological Sciences, University of Bath, Bath BA2 7AY, U.K.;Chemical Defence Establishment, Porton, Salisbury, SP4 0JQ, U.K. |
| |
| Abstract: | S-(+)-3,4-Dihydroxybutylphosphonic acid, an isosteric analogue of sn-glycerol 3-phosphate, was synthesized stereospecifically and shown to be an effective substrate for rabbit muscle glycerol 3-phosphate dehydrogenase (sn-glycerol 3-phosphate-NAD(+) oxidoreductase, EC 1.1.1.8). Non-isosteric phosphonate analogues of sn-glycerol 3-phosphate showed neither substrate nor inhibitory activity with the enzyme. |
| |
| Keywords: | |
|
|
