Features of T-cell subset composition in a D-galactose-induced senescence mouse model |
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Authors: | Koji Kawata Takato Suzuki Kazunori Ozawa Miho Sekiguchi |
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Affiliation: | 1)Laboratory Animal Research Center, Fukushima Medical University, 1 Hikarigaoka, Fukushima 960-1295, Japan;2)Department of Clinical Laboratory Medicine, Fukushima Medical University Hospital, 1 Hikarigaoka, Fukushima 960-1295, Japan;3)Medical-Industrial Translational Research Center, Fukushima Global Science Center, Fukushima Medical University, 1 Hikarigaoka, Fukushima 960-1295, Japan |
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Abstract: | Long-term administration of D-galactose induces oxidative stress and accelerates normal age-related changes. Hence, the D-galactose-treated rodent model has been widely used for aging research. In this study, we examined the immunological characteristics, especially CD4+ T-cell subset composition, of D-galactose-induced aging model mice to evaluate the model’s utility in immunosenescence studies. The spleens of aging model mice subjected to repeated subcutaneous injections of D-galactose exhibited significant increases in T cells with the memory phenotype (CD62Llow CD44high) and individual T-cell subsets (Th1, Th2, Th17 and Treg). Furthermore, cells with the phenotype of T follicular helper (Tfh) cells were spontaneously increased. The features of T-cell subset composition in D-galactose-treated mice were in close agreement with those observed in normal aged mice and appeared to mimic the currently known normal aging processes associated with T-cell homeostasis. Our results suggest that D-galactose-induced aging models would be useful for immunosenescence studies focusing on T-cell homeostasis and give valuable insight into age-related immune system dysregulation. |
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Keywords: | aging model D-galactose immunosenescence T-cell subsets |
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