The binding of bivalent metals by deoxyribonucleic and ribonucleic acids |
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| Authors: | WIBERG J S NEUMAN W F |
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| Affiliation: | 1. Department of Biology, University of Crete, Voutes University Campus, GR-70013, Heraklion, Crete, Greece;2. Department of Chemical Engineering, Cyprus University of Technology, 3603, Limassol, Cyprus;1. Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing, 100176, PR China;2. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, PR China;3. School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing, 100048, PR China |
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| Abstract: | Dissociation constants for DNA and RNA complexes of several bivalent metals have been determined at pH 7.2 by two methods. Equilibrium dialysis has provided KMgDNA = KCaDNA = 8.0 × 10−3 (μ = 0.15); KMgDNA = KCaDNA = 1.2 × 10−2 (μ = 0.20); KMgRNA = KCaRNA = 4.8 × 10−3 (μ = 0.15); KMgRNA = 8.1 × 10−3 (μ = 0.20). The method using a cation-exchange resin has given KCaDNA = 6.11 ± 0.31 × 10−3 and KMnDNA = 3.64 ± 0.28 × 10−3, both at μ = 0.15. The nature of the binding sites has been discussed. The dialyzability and ultraviolet absorbancy of both DNA and RNA have been shown to vary with the type and concentration of salt in solution. |
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