An in vitro technique for the production de novo of multiple shoots in cotyledon explants of cucumber (Cucumis sativus L.) |
| |
Authors: | Rhonda L. Gambley William A. Dodd |
| |
Affiliation: | (1) Centre for Biological Population Management, Queensland University of Technology, GPO Box 2434, Old 4001 Brisbane, Australia |
| |
Abstract: | A technique is described for the production de novo of cucumber (Cucumis sativus L.) shoots in the presence of cytokinin using cotyledon explants. The shoots, which arose from adventitious buds and not from enhanced axillary branching, are confined to a specific region at the base of the cotyledon. Concentrations (4 mgl–1 or less) of the cytokinins 6-benzylaminopurine, kinetin and N6-(2-isopentenyl)adenine, are all effective in producing adventitious buds. It is possible to achieve a yield of 23 shoots per cotyledon by removal of the axillary bud. The yield is increased to 50 shoots per cotyledon by cutting the basal region of the cotyledon into small pieces prior to culturing. These techniques may be useful for transformation studies in cucumber. |
| |
Keywords: | cucumber cotyledon shoot production Cucumis sativus L. adventitious buds |
本文献已被 SpringerLink 等数据库收录! |