Amplification and analysis of human DNA present in mosquito bloodmeals |
| |
| Authors: | RICHARD M. R. COULSON,CHRISTOPHER F. CURTIS,PAUL D. READY&dagger ,NIGEL HILL,DEBORAH F. SMITH |
| |
| Affiliation: | Department of Biochemistry, Imperial College of Science, Technology and Medicine, London;*Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, London;†Entomology Department, The Natural History Museum, London |
| |
| Abstract: | Abstract. DNA fingerprinting should permit the identification of individual human hosts of haematophagous arthropods, providing epidemi-ologically useful information, for example, the biting rates on different people and the impact of insecticide-impregnated bednets.
Investigations reported here demonstrate that it is possible to extract, amplify and fingerprint human DNA from the bloodmeals of individual female Anopheles gambiae mosquitoes kept at 24oC for up to 10–15 h post-ingestion. |
| |
| Keywords: | Anopheles gambiae mosquitoes bloodmeal identification DNA fingerprinting polymerase chain reaction |
|
|
