| 双歧杆菌的DNA对小鼠腹腔巨噬细胞游离Ca2+离子水平的影响 |
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| 引用本文： | 唐铭坚,王立生,朱忠生,马晓东.双歧杆菌的DNA对小鼠腹腔巨噬细胞游离Ca2+离子水平的影响[J].中国微生态学杂志,2005,17(6):403-404. |
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| 作者姓名： | 唐铭坚 王立生 朱忠生 马晓东 |
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| 作者单位： | 1. 深圳市人民医院,广东,深圳,518020
2. 南方医科大学,基础部中心实验室,广东,广州,510515 |
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| 基金项目： | 广东省自然基金资助课题,编号：04006961;深圳市科技局资助课题,编号20030206 |
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| 摘 要： | 目的 从信号转导途径探索青春型双歧杆菌的DNA激活巨噬细胞的机制。方法 用钙离子荧光指示剂Fluo-3／AM负载小鼠腹腔巨噬细胞，以激光共焦镜显微镜观察不同浓度的DNA对巨噬细胞内游离Ca^2＋离子的浓度变化。结果 所用刺激浓度的DNA均能显著升高巨噬细胞内游离Ca^2＋．离子的浓度，并且随着刺激物浓度的逐增，巨噬细胞内游离Ca^2＋离子的上升速度以及达到峰值的荧光值也逐渐升高（P〈0．01）。结论 青春型双歧杆菌的DNA能提高巨噬细胞内游离Ca^2＋离子的浓度，并呈剂量依赖性。
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| 关 键 词： | 双歧杆菌DNA 巨噬细胞 游离Ca^2＋ 离子 激光共焦镜显微镜 |
| 文章编号： | 1005-376X（2005）06-0403-02 |
| 收稿时间： | 2005-07-25 |
| 修稿时间： | 2005年7月25日 |
Influence of DNA of Bifidobatcteria on the free calcium ion level of peritoneal macrophages of mice |
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| TANG Ming-jian,WANG Li-sheng ,ZHU Zhong-sheng ,et al..Influence of DNA of Bifidobatcteria on the free calcium ion level of peritoneal macrophages of mice[J].Chinese Journal of Microecology,2005,17(6):403-404. |
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| Authors: | TANG Ming-jian WANG Li-sheng ZHU Zhong-sheng |
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| Abstract: | Objective The mechanism that DNA of Bifidobacteria adolescence activated macrophages was investigated in view of signal transduction.Methods The macrophages were loaded with Fluo-3/AM.The free calcium ion concentration of macrophages was detected by employing laser scanning confocal microscopy,after the macrophages were stimulated with different dosage of DNA.Results DNA with all different dosage could significantly elevate the free calcium ion concentration of macrophages.With the gradual increment of stimulus dosage,the increasing speed and average fluorescent strength reaching the peak of free calcium ion of the macrophages elevated accordingly(P<0.01).Conclusion DNA of Bifidobacteria adolescence could elevate the free calcium ion concentration of macrophages dose-dependently. |
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| Keywords: | Bifidobactorium DNA Macrophage Free calcium ion Laser scanning confocal microscop |
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