Plant regeneration from encapsulated somatic embryos of pedunculate oak (Quercus robur L.) |
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Authors: | Christine Prewein Eva Wilhelm |
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Institution: | (1) Life Science Department, Austrian Research Centers Seibersdorf, A-2444 Seibersdorf, Austria |
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Abstract: | Summary Cotyledonary Quercus robur L. somatic embryos from two cell lines were encapsulated in 4% (w/v) sodium alginate. An artificial endosperm was provided
by the addition of P24 medium plus 3% (w/v) sucrose. Oak somatic embryos and oak synthetic seeds were germinated on P24 medium plus 0.1 μM indole-3-butyric acid and 0.9 μM 6-benzylaminopurine or were dehydrated prior to germination. The highest conversion rates (26%) were obtained with encapsulated
somatic embryos as well as artificial endosperm-coated somatic embryos. Encapsulation improved the regeneration into oak plantlets
in one of the two cell lines tested. The artificial endosperm had no additional beneficial effect on conversion frequency,
but increased germination rate in one cell line tested. Significant higher conversion could be attributed to slow desiccation
compared to the non-encapsulated control. Cold storage as a post-maturation treatment had no influence on the germination
ability of oak synthetic seeds. Differences in the response of the cell lines with respect to conversion frequencies and timing
of germination were observed. Fifty-six well-developed plantlets regenerated 12 wk after germination, and 29 plants were transferred
to the greenhouse, where they have been successfully established in substrate. |
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Keywords: | artificial seeds forest trees hydrogel beads synseeds |
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