Discs-large (DLG) is clustered by presynaptic innervation and regulates postsynaptic glutamate receptor subunit composition in Drosophila |
| |
Authors: | Kaiyun Chen and David E Featherstone |
| |
Institution: | (1) Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA |
| |
Abstract: | Background
Drosophila discs-large (DLG) is the sole representative of a large class of mammalian MAGUKs, including human DLG, SAP 97, SAP102, and
PSD-95. MAGUKs are thought to be critical for postsynaptic assembly at glutamatergic synapses. However, glutamate receptor
cluster formation has never been examined in Drosophila DLG mutants. The fly neuromuscular junction (NMJ) is a genetically-malleable model glutamatergic synapse widely used to address
questions regarding the molecular mechanisms of synapse formation and growth. Here, we use immunohistochemistry, confocal
microscopy, and electrophysiology to examine whether fly NMJ glutamate receptor clusters form normally in DLG mutants. We
also address the question of how DLG itself is localized to the synapse by testing whether presynaptic innervation is required
for postsynaptic DLG clustering, and whether DLG localization requires the presence of postsynaptic glutamate receptors. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|