| 蔗糖和硝酸铵对黄连悬浮培养细胞生长和小檗碱含量的影响 |
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| 引用本文： | 毛堂芬,颜谦,方周伯. 蔗糖和硝酸铵对黄连悬浮培养细胞生长和小檗碱含量的影响[J]. 生物技术, 1994, 0(3) |
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| 作者姓名： | 毛堂芬 颜谦 方周伯 |
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| 作者单位： | 贵州省农科院生物技术研究所 |
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| 摘 要： | 在６、７－Ｖ液体培养基中较为适合黄连细胞生长的蔗糖浓度为．３％，当蔗糖浓度增加到７％时，细胞生长速率开始下降，但小檗碱含量提高到５．２０％．小檗碱产率为４５６．６０ｍｇ／Ｌ（２３天）。基本培养基中随着补加ＮＨ４ＮＯ３浓度的增加，抑制细胞生长愈严重；补加０．８ｇ／ＬＮＨ４ＮＯ３的小檗碱含量比未加的增加约１０％，随着浓度的增加又急剧下降。
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| 关 键 词： | 黄连；蔗糖；硝酸铵；细胞悬浮培养；小檗碱 |
Effects of Sucrose and Ammonium Nitrate on Cell Growth and Berbrine Content in Cell Suspension culture of Coptis chinensis |
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| Mao Tangfen, Yan Qian, Fang Zhoubo. Effects of Sucrose and Ammonium Nitrate on Cell Growth and Berbrine Content in Cell Suspension culture of Coptis chinensis[J]. Biotechnology, 1994, 0(3) |
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| Authors: | Mao Tangfen Yan Qian Fang Zhoubo |
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| Abstract: | Three per cent of Sucrose in 6. 7-V liquied media was proper concentration for cell growth of Coptis Chinensis. When sucrose concentration was raised to 7 st, cell growth rate bagan to decrease,but berberine content was raised to 5. 20 %, berberine yield was 456. 60mg/L (23 days). When additional NH4NO3 was added to the bassic media, the high the concentration of ammonium nitrate, the more serious the inhibitation on cell growth; The berberine content in the concentration of 0. 8g/LNH4NOs was 10 per cent more than that without it. but berberine content was rapidly decreased followed the concentration increasing of ammonium nitrate. |
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| Keywords: | Coptis chinensis Sucrose Ammonium nitrate Cell suspension culture Berberine |
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