Single-stranded DNA modification by an oligonucleotide-phthalocyanine Fe(II) conjugate: the kinetic features and the process mechanism |
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Authors: | A A Kuznetsova L I Solov’eva O S Fedorova |
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Institution: | (1) Novosibirsk State University, ul. Pirogova 2, Novosibirsk, 630090, Russia;(2) Novosibirsk Institute of Chemical Biology and Fundamental Medicine, Russian Academy of Sciences, pr. Akademika Lavrent’eva 8, Novosibirsk, 630090, Russia;(3) NIOPIK State Scientific Center, ul. Sadovaya 1/4, Moscow, Russia |
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Abstract: | Catalytic oxidative modification of a single-stranded DNA with hydrogen peroxide and molecular oxygen in the presence of a conjugate containing an oligonucleotide complementary to the DNA fragment and tetra-4-carboxyphthalocyanine Fe(II) was studied. The conjugate examined was found to be active in the reaction of oxidative DNA cleavage in the presence of hydrogen peroxide, like the earlier studied oligonucleotide conjugates containing metallocomplexes tetra-4-carboxyphthalocyanine Co(II) and 2,4-di-2-(2-hydroxyethyl)]deuteroporphyrin IX Fe(III) generating active oxygen forms. The new conjugate was more active in the case of oxidation with molecular oxygen. Kinetic features and optimal regimes of DNA oxidation with hydrogen peroxide were found. |
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Keywords: | oligonucleotide-phthalocyanine conjugates oxidative DNA modification |
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