| 蛋氨酸高产酵母突变株的推理筛选 |
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| 引用本文： | 郝勃,付军. 蛋氨酸高产酵母突变株的推理筛选[J]. 微生物学杂志, 1999, 19(1): 6-8 |
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| 作者姓名： | 郝勃 付军 |
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| 作者单位： | 华中农业大学微生物学系!武汉，430070，华中农业大学微生物学系!武汉，430070，华中农业大学微生物学系!武汉，430070，华中农业大学微生物学系!武汉，430070 |
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| 摘 要： | 以啤酒酵母（Saccharomycescerevisiae）菌株Ⅰ为出发株，经紫外线处理后，在含可完全抑制出发株生长的乙硫氨酸（浓度为2g/L）的基本培养从上筛出14株生长良好的突变株，其中I－2，I－9株还能在无硫基本培养基上良好生长。经氨基酸自动分析仪检测，I－2株菌体蛋白中的蛋氨酸含量（质量由分比）由I株的1．93增年9．28。
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| 关 键 词： | 蛋氨酸 乙硫氨酸 抗结构类似物 抗缺硫 |
SCREENING OF METHIONINE HIGH-YIELD YEAST MUTANT BY REASONING MEtTHOD |
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| Hao Bo et al.. SCREENING OF METHIONINE HIGH-YIELD YEAST MUTANT BY REASONING MEtTHOD[J]. Journal of Microbiology, 1999, 19(1): 6-8 |
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| Authors: | Hao Bo et al. |
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| Abstract: | The cells of Saccharomyces cerevisiae strain I was incubated on mineral medium (MM)which conained 2. 0g/L of ethionine after treated with ultraviolet. The concentration ofethionine inhibits the growth of the strain. Fourteen well - growing colonies was screenedfrom the basic medium where could totally inhibit the growth of the starting strain. Reason-ably, they are ethionine resistant mutants. Two of the mutants I - 2 and I - 9 also grew wellin MM lacking sulfur, and I - 2 was confirmed as methionine high - yield strain with auto-matic amino acid analyzer to analyze its amino acid to the whole cell protein. The content ofmethionine increased fon 1. 93 (w/w, % ) of strain I to 9. 28 (w/w, % ). |
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| Keywords: | methionine ethionine structural analogue growth in medium lacking sulfur |
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