Stereospecificity of diacylglycerol for stimulus-response coupling in platelets |
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| Authors: | H Nomura K Ase K Sekiguchi U Kikkawa Y Nishizuka Y Nakano T Satoh |
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| Affiliation: | 1. Department of Diabetes, Endocrinology and Nutrition, Kyoto University Hospital, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan;2. Medical Innovation Center (MIC), Kyoto University Graduate School of Medicine, 53 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan;3. Department of Peptide Research, Kyoto University Graduate School of Medicine, 53 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan;4. Institute for Advancement of Clinical and Translational Science (iACT), Kyoto University Hospital, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan;5. Center for iPS Cell Research and Application (CiRA), Kyoto University, 53 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan;6. Department of Human Health Science, Kyoto University Graduate School of Medicine, 53 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan;1. Laboratory of Physico-Chemistry of Solid State, LR11ES51, Sfax Faculty of Sciences, University of Sfax, Sfax 3071, Tunisia;2. Istituto Nanoscienze-CNR, Via Campi 213a, Modena I-41125, Italy;3. Laboratory for Chemistry of Novel Materials, Université de Mons, Place du Parc 20, Mons 7000, Belgium;4. MMM-UMR 6283 CNRS, LUNAM, Faculty of Sciences and Techniques, University of Maine, Avenue Olivier Messiaen, Le Mans 72085 Cedex 9, France |
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| Abstract: | In intact platelets, a permeable diacylglycerol having a 1,2-sn- but not 2,3-sn- configuration activated protein kinase C directly. In the presence of Ca2+-ionophore this diacylglycerol caused full activation of platelet release reaction. 1,3-Isomer was inactive. Among these isomers only 1,2-sn-diacylglycerol was converted rapidly to the corresponding phosphatidic acid in both intact and broken cell preparations. Thus, the diacylglycerol which functions in stimulus-response coupling possesses a 1,2-sn-glycerol backbone, and other isomers are not involved in the signal transduction through the protein kinase C pathway. |
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