Vitamin B12-binding proteins of the horseshoe crab Limulus polyphemus |
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| Authors: | G M Sharma H T Shigeura L Y Liu |
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| Affiliation: | 1. Vinča Institute of Nuclear Sciences, University of Belgrade, P.O.B. 522, 11001 Belgrade, Serbia;2. The Faculty of Agronomy Čačak, University of Kragujevac, Cara Dušana 34, 32000 Čačak, Serbia;3. Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, P.O. BOX 23, 11010 Belgrade, Serbia;4. University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade, Serbia;5. Serbian Academy of Sciences and Arts, Knez Mihailova 35, 11000 Belgrade, Serbia;6. Polymer Institute, Slovak Academy of Sciences, Dubravska cestá 9, 84541 Bratislava, Slovakia;7. Faculty of Agriculture, Department of Agricultural Engineering, University of Belgrade, Nemanjina 6, Zemun 11080, Serbia;1. Mass Spectrometry in Reactive Flows – Thermodynamics, IVG, University of Duisburg-Essen, 47057 Duisburg, Germany;2. Molecular Dynamics Group, Paul Scherrer Institute, 5232 Villigen PSI, Switzerland;3. Institute of Combustion Technology, German Aerospace Center (DLR), Pfaffenwaldring 38-40, 70569 Stuttgart, Germany;1. University of Toronto, Leslie Dan Faculty of Pharmacy, Toronto, Ontario, Canada;2. University of Wisconsin-Madison, School of Pharmacy, Madison, Wisconsin;3. University of Toronto, Dalla Lana School of Public Health, Toronto, Ontario, Canada |
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| Abstract: | Vitamin B12-binding proteins were detected in the body fluids and/or tissues of horseshoe crabs (Limulus polyphemus), clams and sponges. Among the biological specimens tested the Limulus plasma was especially rich in free B12-binding proteins. Gel filtration experiments revealed that Limulus plasma contains two classes of B12-binding proteins. One class of proteins, molecular weight in excess of 100,000, bind B12 preferentially with affinity constant of 5 X 10(11)M-1. The second type of proteins, molecular weights around 50,000, bind B12 with specificity approaching that of mammalian intrinsic factors. The binding constant of these proteins for B12 is around 10(11)M-1. |
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