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The life history and in vivo culture of Coelomomyces utahensis (Blastocladiomycetes)
Authors:Whisler Howard C  Karanja Diana M Sabwa  Shemanchuk Joseph A  Zebold Stephen L  Romney Steven V  Nielsen Lewis T
Affiliation:a Department of Biology, University of Washington, Seattle, WA 98195, USA
b Center for Vector Biology and Control Research, Kenya Medical Research Institute, P.O. Box 1578 Kisumu, Kenya
c Research Centre, Agriculture and Agri-Food Canada, 1050 Henderson Lake Boulevard, Lethbridge, AB, Canada T1K 3B2
d Uintah County Mosquito Abatement District, 1425 East 1000, South Vernal, UT 84078, USA
e Department of Biology, University of Utah, Salt Lake City Mosquito Abatement District, 2020 North Redwood Road, Salt Lake City, UT 84116, USA
Abstract:Coelomomyces utahensis is a fungal parasite of several genera of mosquitoes that inhabit rock-pools in southern Utah. Studies of the biology of Coelomomyces and their potential use in biological control of mosquitoes have been hindered by their complex life history, lack of axenic culture methods, and logistical problems producing their arthropod hosts for in vivo culture. In the case of C. utahensis, we have identified the alternate microcrustacean host as Potamocypris unicaudata, which is an ostracod that can be easily reared in abundance and stored for long periods. Described here are the life cycle and culturing of C. utahensis.
Keywords:Aedes   Blastocladiomycetes   Coelomomyces utahensis   Culicidae   Mosquito pathogen   Life cycle   Potamocypris unicaudata   Ostracoda
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