Pullulan production by <Emphasis Type="Italic">Aureobasidium pullulans</Emphasis> cells immobilized in chitosan beads |
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Authors: | Thomas P West |
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Institution: | (1) Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA |
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Abstract: | Fungal cells of Aureobasidium pullulans ATCC 201253 were immobilized by entrapment in chitosan beads, and the immobilized cells were investigated for their ability
to produce the polysaccharide pullulan using batch fermentation. The 1% chitosan-entrapped fungal cells were capable of producing
pullulan for two cycles of 168 h using corn syrup as a carbon source. Pullulan production by the immobilized cells increased
by 1.6-fold during the second production cycle (5.0 g/l) relative to the first production cycle (3.1 g/l) with the difference
in production being statistically significant after 168 h. The productivity of the immobilized cells increased during the
second production cycle while its pullulan content decreased. The level of cell leakage from the support remained unchanged
for both production cycles. |
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Keywords: | |
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