The abc1-/coq8- respiratory-deficient mutant of Schizosaccharomyces pombe suffers from glutathione underproduction and hyperaccumulates Cd2+ |
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Authors: | Gazdag Zoltan Fujs Stefan Koszegi Balázs Kálmán Nikoletta Papp Gábor Emri Tamás Belágyi Joseph Pócsi István Raspor Peter Pesti Miklós |
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Institution: | (1) Department of General and Environmental Microbiology, Faculty of Sciences, University of P?cs, 7602 P?cs, P.O. Box 266, Hungary;(2) Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia;(3) Department of Microbial Biotechnology and Cell Biology, Faculty of Sciences, University of Debrecen, Debrecen, Hungary;(4) Department of Biophysics, Medical School, University of P?cs, P?cs, Hungary |
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Abstract: | The abc1
−
/coq8
−
gene deletion respiratory-deficient mutant NBp17 of fission yeast Schizosaccharomyces pombe displayed a phenotypic fermentation pattern with enhanced production of glycerol and acetate, and also possessed oxidative
stress-sensitive phenotypes to H2O2, menadione, tBuOOH, Cd2+, and chromate in comparison with its parental respiratory-competent strain HNT. As a consequence of internal stress-inducing
mutation, adaptation processes to restore the redox homeostasis of mutant NBp17 cells were detected in minimal glucose medium.
Mutant NBp17 produced significantly increased amounts of O2•− and H2O2 as a result of the decreased internal glutathione concentration and the only slightly increased glutathione reductase activity.
The Cr(VI) reduction capacity and hence the •OH production ability were decreased. The mutant cells demonstrated increased specific activities of superoxide dismutases
and glutathione reductase (but not catalase) to detoxify at least partially the overproduction of reactive oxygen species.
All these features may be explained by the decreased redox capacity of the mutant cells. Most notably, mutant NBp17 hyperaccumulated
yellow CdS. |
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Keywords: | |
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