Establishment of an arabinose-inducible system in Stenotrophomonas maltophilia |
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| Authors: | Yi-Wei Huang Rouh-Mei Hu Yu-Ting Chiang Tsao-Chuen Chung Tung-Ching Chung Tsuey-Ching Yang |
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| Affiliation: | (1) Graduate Institute of Microbiology and Public Health, National Chung-Hsing University, Taichung, 402, Taiwan;(2) Department of Biotechnology, Asia University, Taichung, 413, Taiwan;(3) Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, 404, Taiwan;(4) School of Medical Laboratory Science and Biotechnology, Taipei Medical University, Taipei, 110, Taiwan;(5) Department of Veterinary Medicine, National Chung-Hsing University, 250, Kuo Kuang Rd, Taichung, 402, Taiwan, Republic of China;(6) 91 Hsueh-Shih Rd, Taichung, 40402, Taiwan, Republic of China; |
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| Abstract: | A pBBad22T-derived conditioned arabinose (Ara)-inducible expression system was evaluated in Stenotrophomonas maltophilia (an opportunistic pathogen and has gained increasing attention as a cause of healthcare-associated infection). S. maltophilia cannot grow well when Ara is the sole available carbon source. The induction kinetic study, optimal inducer concentration determination, and depletion experiment were performed by using a xylE gene fusion construct, pBxylE, to monitor the expression of pBBad22T in S. maltophilia. For induction survey, the expression of catechol 2,3-dioxygenase (C23O), encoded by xylE gene, continuously increases during an 8-h induced course and can be modulated by different inducer concentrations. The applied induction condition of pBBad22T in S. maltophilia is the inducer concentration ranging from 0.1% to 0.5% for an induction time of 4 h. For repression evaluation, the C23O expression is rapidly turned off within 30 min after the removal of Ara. Accordingly, the established Ara-inducible system can provide a convenient tool for the study of S. maltophilia. |
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