Usefulness of McRAPD for typing and importance of biofilm production in a case of nosocomial ventriculoperitoneal shunt infection caused by <Emphasis Type="Italic">Candida lusitaniae</Emphasis> |
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Authors: | Petr Hamal Juraj Hanzen Frantisek Horn Jitka Trtkova Lenka Ruskova Renata Vecerova Filip Ruzicka Anna Vollekova Vladislav Raclavsky |
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Institution: | (1) Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, Hnevotinska 3, 77515 Olomouc, Czech Republic;(2) Department of Microbiology, Faculty of Medicine and Dentistry and University Hospital, Palacky University Olomouc, Bratislava, Slovak Republic;(3) HPL Ltd. Microbiology Laboratory, Bratislava, Slovak Republic;(4) Department of Pediatric Surgery, Comenius University, Children’s Hospital, Bratislava, Slovak Republic;(5) Institute of Microbiology, Masaryk University Brno, Brno, Czech Republic |
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Abstract: | A case report of ventriculoperitoneal shunt infection caused by Candida lusitaniae in a 6-year-old patient with cerebral astrocytoma and obstructive hydrocephalus is presented briefly with emphasis on the
course of antifungal treatment. Seven isolates recovered subsequently from the cerebrospinal fluid were studied retrospectively.
To confirm identity, isolates were typed using pulsed-field gel electrophoresis and melting curve of random amplified polymorphic
DNA (McRAPD). Further, the ability to form biofilm and its susceptibility to systemic antifungals were evaluated. Using McRAPD,
identity of C. lusitaniae isolates showing slight microevolutionary changes in karyotypes was undoubtedly confirmed; successful application of numerical
interpretation of McRAPD for typing is demonstrated here for the first time. The strain was also recognized as a strong biofilm
producer. Moreover, minimum biofilm inhibitory concentrations were very high, in contrast to low antifungal minimum inhibitory
concentrations of isolates. It can be concluded that McRAPD seems to be a simple and reliable method not only for identification
but also for typing of yeasts. A ventriculoperitoneal shunt colonized by C. lusitaniae was revealed as the source of this nosocomial infection, and the ability of the strain to form biofilm on its surface likely
caused treatment failure. |
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