Dynamics of <Emphasis Type="Italic">Vulmar/VulMITE</Emphasis> group of transposable elements in <Emphasis Type="Italic">Chenopodiaceae</Emphasis> subfamily <Emphasis Type="Italic">Betoideae</Emphasis> |
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Authors: | Dariusz Grzebelus Krzysztof Stawujak Justyna Mitoraj Marek Szklarczyk |
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Institution: | 1.Department of Genetics, Plant Breeding, and Seed Science,University of Agriculture in Krakow,Kraków,Poland |
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Abstract: | Transposable elements are important factors driving plant genome evolution. Upon their mobilization, novel insertion polymorphisms
are being created. We investigated differences in copy number and insertion polymorphism of a group of Mariner-like transposable
elements Vulmar and related VulMITE miniature inverted-repeat transposable elements (MITEs) in species representing subfamily Betoideae. Insertion sites of these elements were identified using a modified transposon display protocol, allowing amplification of longer fragments representing
regions flanking insertion sites. Subsequently, a subset of TD fragments was converted into insertion site-based polymorphism
(ISBP) markers. The investigated group of transposable elements was the most abundant in accessions representing the section
Beta, showing intraspecific insertion polymorphisms likely resulting from their recent activity. In contrast, no unique insertions
were observed for species of the genus Beta section Corollinae, while a set of section-specific insertions was observed in the genus Patellifolia, however, only two of them were polymorphic between P. procumbens and P. webbiana. We hypothesize that Vulmar and VulMITE elements were inactivated in the section Corollinae, while they remained active in the section Beta and the genus Patellifolia. The ISBP markers generally confirmed the insertion patterns observed with TD markers, including presence of distinct subsets
of TE insertions specific to Beta and Patellifolia. |
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