Plant catalase is imported into peroxisomes by Pex5p but is distinct from typical PTS1 import |
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Authors: | Oshima Yoshimi Kamigaki Akane Nakamori Chihiro Mano Shoji Hayashi Makoto Nishimura Mikio Esaka Muneharu |
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Institution: | 1Graduate School of Biosphere Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima, 739-8528 Japan
2Department of Cell Biology, National Institute for Basic Biology, Okazaki, 444-8585 Japan |
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Abstract: | We have previously demonstrated that the targeting signal ofpumpkin catalase, Cat1, is an internal PTS1 (peroxisomal targetingsignal 1)-like sequence, QKL, located at –13 to –11from the C-terminus, which is different from the typical PTS1SKL motif located in the C-terminus. Here we show that Cat1import into peroxisome is dependent on the cytosolic PTS receptor,Pex5p, in Arabidopsis, similar to typical PTS1 import, and thatother components for transport of peroxisomal matrix proteinssuch as Pex14p, Pex13p, Pex12p and Pex10p also contribute tothe import of Cat1. Interestingly, however, we found that Cat1interacts with the N-terminal domain of Pex5p, but not the C-terminaldomain for interaction with the typical PTS1, revealing thatPex5p recognizes Cat1 in a manner distinct from typical PTS1. |
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Keywords: | Arabidopsis - Catalase - Peroxisome - Peroxisomal targeting signal - PEX - Two-hybrid system |
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