| 使用超净工作台实施子宫摘除术净化KM小鼠的研究 |
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| 引用本文： | 卢笑丛,徐国景,戴涌,唐利军,孙凡中,余思义,张金明,田辉,张燕,张春. 使用超净工作台实施子宫摘除术净化KM小鼠的研究[J]. 中国实验动物学报, 2003, 11(4): 208-210 |
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| 作者姓名： | 卢笑丛 徐国景 戴涌 唐利军 孙凡中 余思义 张金明 田辉 张燕 张春 |
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| 作者单位： | 1. 中国科学院武汉植物研究所/武汉植物园,武汉,430079
2. 湖北省预防医学科学院,武汉,430079 |
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| 基金项目： | 湖北省科技厅"九五"重点科技发展项目(编号972P0904) |
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| 摘 要： | 目的建立SPF级湖北封闭群KM小鼠生产群.方法使用超净工作台实施子宫摘除术净化小鼠的方法,建立了SPF级湖北封闭群KM小鼠生产群,并对子一代进行了微生物及寄生虫的检测.结果使用上述方法实施手术67例,成功57例,得净化仔鼠509只,育成的460只小鼠符合SPF级标准.结论使用生物净化的方法可以保存优良实验动物品种.
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| 关 键 词： | 小鼠,KM 净化 子宫切除术 |
| 文章编号： | 1005-4847(2003)04-0208-03 |
| 修稿时间： | 2002-10-29 |
Study on the Purification of Kunming Mouse Strain by Hysterectomy in the Situation of Bacteria Free |
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| LU Xiao-cong,XU Guo-jing,DAI Yong,TANG Li-jun,SUN Fan-zhong,YU Si-yi,ZHANG Jin-ming,TIAN Hui,ZHANG Yan,ZHANG Chun. Study on the Purification of Kunming Mouse Strain by Hysterectomy in the Situation of Bacteria Free[J]. Acta Laboratorium Animalis Scientia Sinica, 2003, 11(4): 208-210 |
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| Authors: | LU Xiao-cong XU Guo-jing DAI Yong TANG Li-jun SUN Fan-zhong YU Si-yi ZHANG Jin-ming TIAN Hui ZHANG Yan ZHANG Chun |
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| Affiliation: | LU Xiao-cong1,XU Guo-jing2,DAI Yong2,TANG Li-jun2,SUN Fan-zhong2,YU Si-yi2,ZHANG Jin-ming2,TIAN Hui2,ZHANG Yan2,ZHANG Chun2 |
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| Abstract: | Objective To establish special pathogen free Kunming mouse of Hubei closed colony.Method The purification mouse was made by excising operation of uterus in the situation of bacteria free. The SPF Kunming mouse strain of Hubei closed colony was developed with the following surveillance on microbes and parasites in first generation.Results 57 mice were got from 67 cases by operation, and 509 baby mice with purification,from which 460 grew into adults and the state of microbiology in those mice was in keeping SPF.Conclusion The method can keep the excellent laboratory animal stain with biological purification. |
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| Keywords: | Mice KM Purification Hysterectomy |
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