The role of exogenous iron in the activation of ribonucleotide reductase from Escherichia coli |
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Authors: | Jacques Covès Jean-Pierre Laulhère M Fontecave |
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Institution: | (1) Laboratoire d'Etudes Dynamiques et Structurales de la Sélectivité, Unité Mixte de Recherche du Centre National de la Recherche Scientifique no. 5616, Université Joseph Fourier, BP 53, F-38041 Grenoble Cédex 9, France, FR |
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Abstract: | Protein R2, the small component of ribonucleotide reductase from Escherichia coli, contains a diferric center and a catalytically essential tyrosyl radical. In vitro, this radical can be produced in the
protein from two inactive forms, metR2, containing an intact diiron center and lacking the tyrosyl radical, and apoR2, lacking
both iron and the radical. While activation of apoR2 requires only a source of ferrous iron and exposure to O2, activation of metR2 was achieved using a multienzymatic system consisting of an NAD(P)H:flavin oxidoreductase, superoxide
dismutase and a poorly defined protein fraction, named fraction b (Fontecave M, Eliasson R, Reichard P (1987) J Biol Chem
262 : 12325–12331). In both reactions, reduced R2, containing a diferrous center, is a key intermediate which is subsequently
converted to active R2 during reaction with O2. By in vivo labeling of E. coli with radioactive 59Fe, we show that fraction b contains iron. Depletion of the iron in fraction b inactivates it, and fraction b can be substituted
for by ferric citrate solutions. Furthermore, aqueous Fe2+ in the presence of dithiothreitol is able to convert metR2 into reduced R2. Therefore we propose that the function of fraction
b is to provide, in association with the flavin reductase, ferrous iron for reduction of the endogenous diiron center. Since
fraction b is not a single well-defined protein, it remains to be shown whether, in vivo, that function resides in a specific
protein. Exogenous iron can thus participate in activation of both apoR2 and metR2, but it is incorporated into R2 only in
the former case. A unifying mechanism is proposed.
Received: 13 November 1996 / Accepted: 3 April 1997 |
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Keywords: | Ribonucleotide reductase Iron metabolism Escherichia coli |
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