IGF-I induces caveolin 1 tyrosine phosphorylation and translocation in the lipid rafts |
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Authors: | Maggi Davide Biedi Claudia Segat Daniela Barbero Daniela Panetta Danilo Cordera Renzo |
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Institution: | Department of Endocrinology and Metabolism, University of Genoa, Genoa, Italy. |
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Abstract: | Caveolin 1, a component of caveolae, regulates signalling pathways compartmentalization interacting with tyrosine kinase receptors and their substrates. The role of caveolin 1 in the Insulin Receptor (IR) signalling has been well investigated. On the contrary, the functional link between caveolin 1 and IGF-I Receptor (IGF-IR) remains largely unknown. Here we show that (1) IGF-IR colocalizes with caveolin 1 in the lipid rafts enriched fractions on plasmamembrane in R-IGF-IR(WT) cells, (2) IGF-I induces caveolin 1 phosphorylation at the level of tyrosine 14, (3) this effect is rapid and results in the translocation of caveolin 1 and in the formation of membrane patches on cell surface. These actions are IGF-I specific since we did not detect caveolin 1 redistribution in insulin stimulated R(-) cells overexpressing IRs. |
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Keywords: | IGF-I receptor Insulin receptor Caveolin 1 Lipid raft Tyrosine phosphorylation |
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