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Cloning and overexpression of Moloney murine leukemia virus reverse transcriptase in Escherichia coli |
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| Authors: | M L Kotewicz J M D'Alessio K M Driftmier K P Blodgett G F Gerard |
| Affiliation: | Bethesda Research Laboratories Life Technologies Inc. Molecular Biology Research and Development Gaithersburg, MD 20877 U.S.A. Tel. (301) 670-8341 |
| Abstract: | |
| Keywords: | Recombinant DNA phage λ vector reconstruction of the ATG codon active enzyme folding aa amino acid(s) AMV avian myeloblastosis virus Ap ampicillin bp base pair(s) BSA bovine serum albumin buffer A see MATERIALS AND METHODS, section e DTT dithiothreitol EtBr ethidium bromide IgG immunoglobulin G kb 1000 bp M-MLV Moloney murine |
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