A Physical Map of the Genome of a Unicellular Cyanobacterium Synechocystis sp. Strain PCC6803 |
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Authors: | Kotani, Hirokazu Kaneko, Takakazu Matsubayashi, Toru Sato, Shusei Sugiura, Masahiro Tabata, Satoshi |
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Affiliation: | 1Kazusa DNA Research Institute Yanauchino, Kisarazu, Chiba 292, Japan 2Center for Gene Research, Nagoya University Furoh-Cho, Nagoya 464-01, Japan |
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Abstract: | An accurate physical map of the genome of a cyanobacterium,Synechocystis sp. strain PCC6803, was constructed on the basisof restriction and linking clone analysis. The genome contained6 recognition sites for AscI, 25 sites for MluI, and 31 sitesfor SplI, and the entire genome size was estimated to be 3.6Mb. Sixteen genes or gene clusters, including those involvedin the photosynthetic systems, were localized on the physicalmapof the genome by hybridization. In the course of the above analysis,two extra chromosomal units with approximate sizes of 110 kband 125 kb were identified. |
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Keywords: | Synechocystis PCC6803 physical map of genome restriction analysis linking clone analysis photosynthetic genes |
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