Induction and Secretion of the Chemokines Interleukin-8 and Monocyte Chemotactic Protein-1 in Human Immature Leukemia Cell Lines |
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| Institution: | 1. Chirurgie plastique reconstructrice et esthétique, hôpital de la Croix-Rousse, 103, Grande rue de la Croix-Rousse, 69004 Lyon, France;2. Chirurgie plastique reconstructrice et esthétique, clinique du Parc Lyon, 155, boulevard de Stalingrad, 69006 Lyon, France;1. Departamento de Ingeniería Química y Procesos de Minerales, Facultad de Ingeniería, Universidad de Antofagasta, Antofagasta 1240000, Chile;2. Faculty of Engineering and Architecture, Universidad Arturo Prat, Almirante Juan José Latorre 2901, Antofagasta 1240000, Chile;3. Escuela de Ingeniería Química, Pontificia Universidad Católica de Valparaíso, Valparaíso 2340000, Chile;1. Faculty of Medicine, University of New South Wales, Kensington, NSW, Australia;2. Sydney Medical School Nepean, The University of Sydney, Penrith, NSW 2750, Australia;3. Department of Geriatric Medicine, Nepean Hospital, Penrith, NSW 2750, Australia;4. Research Group on Geriatrics and Gerontology, Faculty of Health Sciences, International Association of Gerontology and Geriatrics Collaborative Centre, University of Caldas, Manizales, Colombia;5. Australian Institute for Musculoskeletal Science (AIMSS), The University of Melbourne and Western Health, St. Albans, VIC 3021, Australia;6. Department of Medicine, Melbourne Clinical School – Western Campus, St. Albans, VIC 3021 Australia;1. Advanced Nanoengineering Materials Laboratory, Materials Science Programme, Indian Institute of Technology Kanpur, Kanpur 208016, India;2. Advanced Nanoengineering Materials Laboratory, Department of Mechanical Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, India;1. Department of Hematology, Affiliated Hospital of Chengde Medical University, Chengde, Hebei, China;2. Department of Scientific Research, Affiliated Hospital of Chengde Medical University, Chengde, Hebei, China;1. The Institute of Environmental Toxicology, 4321, Uchimoriya-machi, Joso-shi, Ibaraki, 303-0043, Japan;2. Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Azabu University, 1-17-71, Fuchinobe, Chuo-ku, Sagamihara, Kanagawa, 252-5201, Japan |
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| Abstract: | We investigated expression and secretion of the chemokines interleukin-8 (IL-8) and monocyte chemotactic protein-1 (MCP-1) in human myeloid cell lines. Quantitative determination by ELISA revealed a significant constitutive production of both chemokines in the cell lines HL-60 and NB-4 (>1000 pg/ml IL-8 and >400 pg/ml MCP-1 per million cells), while in the cell lines EOL-1, KASUMI-1 and KG-1 only 10–100 pg/ml IL-8 and MCP-1 were detected. Tetradecanoyl phorbol acetate (TPA) strongly increased the IL-8 and MCP-1 amounts in the culture supernatants of all five cell lines. The TPA-induced NB-4 produced the largest amounts of both chemokines (>40,000 pg/ml). The strongest induction was seen in EOL-1 (>100-fold increase). Besides TPA, tumor necrosis factor-α (TNFα) also distinctively enhanced IL-8 and MCP-1 production. The calcium ionophore A-23187 and thapsigargin, an inhibitor of the Ca2+-ATPase, differentially induced IL-8 and MCP-1 secretion in the cell lines investigated, suggesting that, at least in some cell lines, intracellular free Ca2+ might be important for chemokine secretion. Dexamethasone significantly prevented the IL-8 and MCP-1 production of stimulated cells, emphasizing the potent anti-inflammatory property of glucocorticoids. Similarly, the protein kinase inhibitor staurosporine clearly decreased the TPA-induced chemokine secretion in NB-4 cells, indicating the involvement of protein kinases in the signal transduction pathway which leads to enhanced chemokine secretion. |
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