Early Responses in Methyl Jasmonate-Preconditioned Cells toward Pathogen-Derived Elicitors |
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| Institution: | 1. CITAB - Centre for the Research and Technology of Agro-Environmental and Biological Sciences, Universidade de Trás-os-Montes e Alto Douro, 5000-801, Vila Real, Portugal;2. Chemistry Department, University of Trás-os-Montes and Alto Douro, 5000-801, Vila Real, Portugal;3. CIMO - Mountain Research Centre, Polytechnic Institute of Bragança, 5300-253, Bragança, Portugal;1. Department of Plant Physiology, Facultad de Ciencias, University of Granada, Fuentenueva s/n, 18071 Granada, Spain;2. Department of Biology and Geology, Escuela Superior de Ingeniería, University of Almería, La Cañada de San Urbano s/n, 04120 Almería, Spain;1. Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran;2. Department of Biology, Faculty of Science, University of Maragheh, Maragheh, Iran;3. Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran;1. Faculty of Commerce, Fukuoka University, Fukuoka 814-0180, Japan;2. Department of Economics and Finance, Southeast Missouri State University, Cape Girardeau, MO 63701, USA;3. University of Missouri, Columbia, MO, USA;1. Phytochemistry Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, 226015, India;2. Enzyme and Microbial Biochemistry Laboratory, Department of Chemistry, Indian Institute of Technology, Delhi, India;3. Microbiology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, 226015, India;4. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, 201002, India |
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| Abstract: | Early, signal transduction-related responses in cultured tobacco cells due to methyl jasmonate (MeJa), a cell-wall-derived elicitor from Phytophthora nicotianae and chitosan, were investigated. MeJa was an effective inducer of lipid peroxidation and lipoxygenase (LOX) activity with maximum levels reached within 2 h and 4–8 h, respectively. Chitosan and the elicitor induced a transient increase (1–4 h) in lipid peroxidation. Conditioning with MeJA, followed by secondary elicitation, led to a significant increase in malondialdehyde concentration after 1 h. Chitosan and the elicitor induced transient activation of LOX with maximal values between 8 and 12 h, with preconditioning resulting in a rapid increase in LOX activity at 4 h post elicitation. MeJA did not effect phosphoprotein accumulation but conditioning led to the potentiation and differential induction of phosphoproteins due to chitosan and elicitor. The results indicate that cells are sensitized by the exposure to MeJa to respond more intensely and rapidly toward secondary elicitation by fungal pathogen derived elicitors. |
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