Differential Gene Expression in Synovium of Rheumatoid Arthritis and Osteoarthritis |
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| Institution: | 1. Clinic of Orthopedics, University of Regensburg, Bavarian Red Cross Hospital for Rheumatic Diseases, Bad Abbach, Germany;2. Medizinische Poliklinik, University of Bonn, Bonn, Germany;3. Department of Immunology and Cell Biology, Institute of Experimental Dermatology, University of Münster, Münster, Germany;1. University of New Brunswick, 100 Tucker Park Road PO Box 5050, Saint John, NB, E2L 4L5, Canada;2. Dalhousie University, 100 Tucker Park Rd, PO Box 5050, Saint John, NB, E2L 4L5, Canada;3. Horizon Health Network, 560 Main Street, Building A, Suite 325, Saint John, NB, E2K 1J5, Canada;1. School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India;2. School of Life Sciences, Jawaharlal Nehru University, New Delhi, India;1. Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA, 02118, USA;2. Center for Thrombosis and Hemostasis, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany;3. Center for Cardiology, Cardiology I, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany;1. Department of Genetics & Biotechnology, Bhavan''s Vivekananda College of Science, Humanities and Commerce, Sainikpuri, Secunderabad 500094, Telangana State, India;2. Department of Genetics, Osmania University, Hyderabad 500007, Telangana State, India;3. Division of Biostatistics, National Institute of Nutrition, Hyderabad 500007, Telangana State, India |
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| Abstract: | Rheumatoid arthritis (RA) and osteoarthritis (OA) are the major types of arthritis. Although both diseases are characterized by joint destruction, their etiologies are different. To get insights into pathophysiological pathways, we used the suppression subtractive hybridization (SSH) method to identify differentially expressed genes in RA. DNA sequencing identified 12 gene products including cytoskeletal γ-actin and extracellular matrix components such as fibronectin, collagen IIIα1, and superficial zone protein. Interferon γ-inducible genes such as a novel thiol reductase, two genes of unknown function (HSIFNIN4, RING3), and annexin II were also found. Two genes encoded proteins involved in proliferation such as elongation factor 1α and the granulin precursor. Furthermore, the protease cathepsin B and synovial phospholipase A2 group IIA were detected by SSH. To confirm the differential expression of the genes, we performed RT-PCR analyses of RA and OA synovial tissues. Compared to OA patients, 9 of the 12 genes were overexpressed in RA, suggesting that SSH is a powerful tool for the detection of differential gene expression in synovial tissues. Further characterization of the gene products may help to identify pathophysiological mechanisms in arthritic diseases. |
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