Changes in protein synthesis and acid tolerance in Clostridium perfringens type A in response to acid shock. |
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| Authors: | L Villarreal N L Heredia S García |
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| Institution: | Faculty of Biological Sciences, Autonomous University of Nuevo León, San Nicolás, México. |
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| Abstract: | The induction of acid-shock proteins and the degree of acid resistance conferred on Clostridium perfringens by acid shock, and the kinetics of this resistance were determined. A sublethal acid shock at pH 4.5 for 20 min increased the acid tolerance of cells at least fifteenfold. The acquired tolerance was maintained for 3 h after acid treatment. The response of the microorganism to acid shock was also examined by analysis of pulse-labeled proteins. Five acid-shock proteins (molecular weights 120, 84, 58, 45 and 17 kDa) were identified by polyacrylamide gel electrophoresis. |
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