Simultaneous on line monitoring of intracellular β-galactosidase activity and biomass using flow injection analysis inEscherichia coli batch fermentations |
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| Authors: | F Valero F J Lafuente C Solà A Benito M Vidal J Cairó A Villaverde |
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| Institution: | (1) Instituto de Ingeniería Bioquímica (CSIC-UAB), Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain;(2) Institut de Biologia Fonamental i Departament de Genética i Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain |
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| Abstract: | Summary A novel method to monitor on-line intracellular β-galactosidase activity and biomass simultaneously, using flow injection
analysis (FIA), has been developed. The automatic ultrasonic cell disruption and FIA analysis allow the processing of 10 samples/hour
with a wide and variable linear working range of β-galactosidase activity and biomass and a maximum relative standard deviation
(RSD) of 1.5%. The system has been optimized by monitoring biomass and intracellular β-galactosidase activity inEscherichia coli batch fermentation. |
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