Yew (Taxus x media Rehd.) cell suspension cultures as a source of taxanes |
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Authors: | Matja? Hren ?pela Baebler Marjana Camloh Maja Kova Maja Ravnikar Jana ?el |
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Institution: | (1) Department of Plant Physiology and Biotechnology, National Institute of Biology, Ve na pot 111, 1000 Ljubljana, Slovenia |
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Abstract: | Natural resources of paclitaxel, an effective anticancer compound, were threatened with extinction soon after the discovery
of this valuable substance. Cell suspension cultures derived from different Taxus species have rapidly become an alternative source of paclitaxel and other taxanes. In this paper we provide some insight
into cell growth characteristics in cell suspension culture of Taxus x media cv. Hicksii, with emphasis on the effects of jasmonic acid (JA) on taxane production in cell lines with different initial taxane content.
Additionally cell growth characteristics of two cell lines was followed during cultivation of cell suspension culture of Taxus x media cv. Hicksii. Packed cell volume (PCV) was shown to be a reliable and efficient alternative for measuring cell growth instead of fresh
and dry weight. The initial total taxane content was screened in a number of cell lines, followed by observing the effect
of JA on cell mass and total taxane production of selected lines. We showed a great variability in initial taxane content
in different cell lines, which decreased during cell suspension maintenance. JA was shown to inhibit cell growth and increase
total taxane production (14 to 106 fold). |
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Keywords: | tissue culture jasmonic acid taxol Taxus x media cv Hicksii |
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