RNA interference-mediated silencing of the syntaxin 5 gene induces Golgi fragmentation but capable of transporting vesicles |
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Authors: | Suga Kei Hattori Hiroshi Saito Ayako Akagawa Kimio |
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Affiliation: | Department of Cell Physiology, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan. ksuga@kyorin-u.ac.jp |
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Abstract: | It has been suggested that syntaxin 5 (Syx5) participates in vesicular transport. We examined the effects of Syx5 down-regulation on the morphology of the Golgi apparatus and the transport of vesicles in mammalian cells. Knockdown of the Syx5 gene resulted in Golgi fragmentation without changing the level of endoplasmic reticulum (ER)-resident proteins, other Golgi-SNAREs (soluble N-ethylmaleimide-sensitive factor-attachment protein receptors), and coatmer proteins. Strikingly, a major decrease in Syx5 expression barely affected the anterograde transport of vesicular stomatitis virus G (VSVG) protein to the plasma membrane. These results suggest that Syx5 is required for the maintenance of the Golgi structures but may not play a major role in the transport of vesicles carrying VSVG between the ER and the Golgi compartment. |
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Keywords: | ELISA, enzyme-linked immunosorbent assay ER, endoplasmic reticulum ERGIC/VTC, ER-Golgi-intermediate compartment/vesicular tubular carriers GFP, green fluorescent protein GST, glutathione-S-transferase HA, hemagglutinin mAb, monoclonal antibody RNAi, RNA interference siRNA, small interfering RNA SNARE, soluble N-ethylmaleimide-sensitive factor-attachment protein receptor Syx, syntaxin VSVG, vesicular stomatitis virus ts045 G |
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