Electroporator with automatic change of electric field direction improves gene electrotransfer in-vitro |
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Authors: | Matej Reberšek Cécile Faurie Maša Kandušer Selma Čorović Justin Teissié Marie-Pierre Rols Damijan Miklavčič |
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Affiliation: | (1) Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, SI-1000 Ljubljana, Slovenia;(2) Institut de Pharmacologie et de Biologie Structurale du CNRS UMR 5089, 205, route de Narbonne, 31077 Toulouse cedex, France |
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Abstract: | Background Gene electrotransfer is a non-viral method used to transfer genes into living cells by means of high-voltage electric pulses. An exposure of a cell to an adequate amplitude and duration of electric pulses leads to a temporary increase of cell membrane permeability. This phenomenon, termed electroporation or electropermeabilization, allows various otherwise non-permeant molecules, including DNA, to cross the membrane and enter the cell. The aim of our research was to develop and test a new system and protocol that would improve gene electrotransfer by automatic change of electric field direction between electrical pulses. |
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