Batch production of Pyranose 2-oxidase from <Emphasis Type="Italic">Trametes versicolor</Emphasis> (ATCC 11235) in medium with a lignocellulosic substrate and enzymatic bleaching of cotton fabrics |
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Authors: | Nurdan Kasikara Pazarlioglu Emre Erden M Cigdem Ucar Alper Akkaya A Merih Sariisik |
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Institution: | (1) Faculty of Science, Biochemistry Department, Ege University, 35100 Bornova, Izmir, Turkey;(2) Faculty of Engineering, Textile Engineering Department, Dokuz Eylul University, Buca, Izmir, Turkey |
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Abstract: | The aim of this work was to determine new, different and low-cost substrates that can be used for enzyme production from the
white rot fungus Trametes versicolor (ATCC 11235) by taking advantage of the broad substrate specificity of pyranose 2-oxidase. In this report, we investigated
the production of pyranose 2-oxidase from T. versicolor (ATCC 11235) using ten different agricultural residues such as clover straw, almond shells, hazelnut cobs, grass and others.
Pyranose 2-oxidase activity was determined as 2.332 U/g at the 9th day in a submerged culture containing clover straw and
tap water shaken at 150 rpm and 26°C, and the optimum clover straw concentration was determined to be 12 g/l. The effects
of different glucose, nitrogen and phosphate sources on the production of pyranose 2-oxidase were studied in the clover straw
medium. Analyses of biomass, protein, reduced sugar and nitrogen concentrations were also monitored in a clover straw medium
that did not contain carbon or nitrogen and phosphate sources under the parameters determined. The produced pyranose 2-oxidase
was used for improving the properties of cotton fabrics. |
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