Interaction of Delta Sleep-inducing Peptide and Valproate on Metaphit Audiogenic Seizure Model in Rats |
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Authors: | Olivera Stanojlović Dragan Hrnčić Aleksandra Rašić Helena Lončar-Stevanović Dragan Djuric Veselinka Šušić |
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Institution: | (1) Institute of Physiology, School of Medicine, University of Belgrade, Višegradska 26/II, Belgrade, 11000, Serbia;(2) Serbian Academy of Sciences and Arts, Belgrade, 11000, Serbia |
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Abstract: | Effects of valproate (VPA), a conventional antiepileptic drug and natural delta sleep-inducing peptide (DSIP) on metaphit
(1-1-(3-isothiocyanatophenyl)-cyclohexyl]-piperidine)-induced audiogenic reflex epilepsy were studied. For the purpose of the study, valproate in the doses of 50 or 75 mg/kg and
DSIP (1.0 mg/kg) was i.p. injected either alone or in combination to adult Wistar male rats with fully developed metaphit
seizures after eight audiogenic testing. The animals were stimulated using an electric bell (100 ± 3 dB and 5–8 kHz, for 60 s)
60 min after metaphit injection and afterwards at hourly intervals during the experiment. For EEG recording and power spectra
analysis, three gold-plated screws were implanted into the scull. In EEGs of metaphit-treated animals polyspikes, spike-wave
complexes and sleep-like patterns were recorded, while the power spectra were increased. Combined treatment of metaphit-induced
seizures with valproate and DSIP was more effective than drugs alone especially during 4 h after administration. None of the
applied dose combinations eliminated the EEG signs of metaphit-provoked epileptiform activity. Taken together, the results
of the present study suggest that the combinations of valproate and DSIP should be considered as beneficial polytherapy in
metaphit model of epilepsy. |
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Keywords: | Audiogenic seizures DSIP Metaphit Rat Valproate |
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