Inhibition of gastric mucosal laminin receptor by Helicobacter pylori lipopolysaccharide. |
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Authors: | B L Slomiany J Piotrowski S Sengupta A Slomiany |
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Affiliation: | Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2400. |
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Abstract: | Laminin receptor was isolated from gastric epithelial cell membrane by the procedure involving membrane solubilization with octylglucoside followed by affinity chromatography on laminin-coupled Sepharose. The receptor protein, eluted from the matrix with cation-free EDTA buffer, yielded on SDS-PAGE a single 67kDa band. After radioiodination, the protein was incorporated into liposomes which displayed specific affinity toward the laminin-coated surface. The binding of liposomal receptor to the laminin-coated surface was inhibited by lipopolysaccharide from H.pylori. The inhibitory effect was proportional to the concentration of lipopolysaccharide up to 50 micrograms/ml at which point a 96% decrease in the receptor binding occurred. It is suggested that a similar process may account for the loss of mucosal integrity in the pathogenesis of H. pylori associated gastric disease. |
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