Heat-sensitive DNA-binding activity of thecI product of bacteriophage lambda |
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| Authors: | N C Mandal and M Lieb |
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| Institution: | (1) Department of Microbiology, University of Southern California School of Medicine, 90033 Los Angeles, California, USA;(2) Present address: Department of Biochemistry, Bose Institute, 9 Calcutta, India |
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| Abstract: | Summary The binding of  genecI product to DNA was studied at temperatures from 0°C to 46° C. Binding activity of the products ofcIts mutants was higher at 22° C than at 0° C, 26° C or 30° C. BothcI+ andcIts products lost DNA-binding activity at 46° C, but after subsequent cooling to 22° C, they regained 50–100% of their activity. |
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