Glycoproteins that distinguish different cell types found in amniotic fluid

Summary Two concanavalin A-binding glycoproteins of approximate Mw 150 K and 140 K are described, which enable two of the main cell types found in amniotic fluid samples obtained by amniocentesis, AF and F cells, to be reliably distinguished. Further, the Mw 150 K glycoprotein has been used to trace the developmental origin of the AF cell type. AF cells, which generally form the majority of amniotic fluid cells found in culture, appear to arise in the extra-embryonic membranes, specifically the amnion.