Enhanced Polymer One-Step Staining for Proliferating Cell Nuclear Antigen in Squamous Cell Carcinoma of the Tongue |
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| Authors: | Kazuo Sano Joji Sekine Tsugio Inokuchi Michael B Pe Guowu Ma |
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| Institution: |
a Second Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, Sakamoto, Nagasaki, Japan |
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| Abstract: | A rapid enhanced polymer one-step staining for proliferating cell nuclear antigen EPOS-PCNA) in formalin fixed, paraffin embedded sections of tongue squamous cell carcinoma is described. EPOS-PCNA was compared with PCNA immunohistochemistry using the avidin-biotin complex (ABC) method, with or without autoclave pretreatment. Significant correlation of PCNA labeling index (percentage of labeled cells/total cells counted) was observed between EPOS-PCNA and the immunohistochemical protocol using autoclave pretreatment. We consider this method a reliable, timesaving technique that would be useful in quantitative histopatbology and in performing double immunostaining. |
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| Keywords: | antigen retrieval autoclave immunohistochemistry PCNA rapid staining tongue cancer |
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