Phylogenetic identification of epibiotic bacteria possessing antimicrobial activities isolated from red algal species of Japan |
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Authors: | Manmadhan Kanagasabhapathy Hideaki Sasaki Shinichi Nagata |
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Institution: | (1) Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457, Zhongshan Road, Dalian, 116023, China;(2) Department of Life and Environment Science, College of Science and Engineering, Iwaki Meisei University, 5-5-1, Chuodaiiino, Iwaki Fukushima, 970-8551, Japan;(3) Environmental Biochemistry Group, Research Center for Inland Seas, Kobe University, 5-1-1 Fukaeminamicho, Higashinada-ku Kobe, 658-0022, Japan |
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Abstract: | We investigated the diversity of epibiotic bacteria possessing antimicrobial activity isolated from nine species of red algae,
and identified their phylogenetic position. For the isolation of epibiotic bacteria, nine species of red algae, Pachymeniopsis lauceolata, Plocamium telfairiae, Gelidium amansii, Chondrus oncellatus, Grateloupia filicina, Ceramium kondoi, Lomentaria catenata, Schizymenia dubyi and Porphyra yezoensis, were collected from the intertidal zone of Awaji Island, Japan. In total 92 bacteria were collected from the above red algal
species. Primary screening results using disc diffusion assay revealed that 33% of bacteria possess antibacterial activity.
Ten bacteria that showed high antibacterial activity were further studied for their ability to inhibit a set of fouling bacteria,
some luminescent Vibrio and Photobacterium species and a panel of pathogenic bacteria. In general, the inhibitory activities were high against fouling and luminescent
bacteria, while low against various pathogenic bacteria tested. These results suggest that some epibiotic bacteria have adapted
to defend their position in their surface environment through the production of antibacterial metabolites giving defense against
a broad spectrum of bacterial competitors. The phylogenetic analysis using 16 S rRNA sequences identified 7 of the 10 strains
as belonging to the genus Bacillus, and other strains each 1 belonging to genus Microbacterium, Psychrobacter, and Vibrio species. |
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Keywords: | Red algae Marine epibiotic bacteria Antimicrobial activity Fouling bacteria |
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